MRDNA 16s DNA Sequencing Service

Amplicon Sequencing (bTEFAP®)

Sequencing Applications

Barcoded amplicon sequencing has become a popular sequencing solution for scientists interested in a specific genomic region.  Amplicon sequencing combined with next-generation sequencing allows for thousands of amplicons across many samples to be prepared simultaneously and indexed within hours and often within a single-run. Due to its popularization, barcoded amplicon sequencing can now be completed through a variety of different processes utilizing several different technologies. MR DNA has been at the forefront of this technology and through calculated fine tuning, we are proud to offer an inexpensive sequencing method known as bTEFAP®. Originally designed to employ 16s eubacterial primers to be sequenced via pyrosequencing technology, we have expanded its low cost sequencing capabilities to include Archaea, Fungi, and functional gene primers compatible to be sequenced across all of our sequencing platforms. The increased flexibility and versatility of bTEFAP® gives us the opportunity to provide our customers with the lowest sequencing cost possible.

 

Amplicon Sequencing Prices

1)    2x300bp Illumina:  (Price per sample can be lower, depending on how many samples, more samples = lower price per sample) .. you can of course request greater depth. (we can take smaller projects but it may be small delay as we gather enough samples to coordinate with small projects).   We can currently have a variety of 16s assays, fungi, archaea and a few functional genes.. Our system allows us to inexpensively setup new assays and custom assays also

 

2) 5,000 avg sequence per sample full length 16s sequencing. Projects less than 10 samples may include an additional library fee. Please contact us for more details.

 

3)    3,000 avg sequence per sample pyrosequencing  (most commonly requested) any size project wide variety of assays available.   Good general overview of microbiome. Good for taxonomy and alpha and beta diversity.  A wide variety of assays are currently available for 16s bacteria, archaea, fungi, functional genes etc.

 

4)    10,000 avg sequence pyrosequencing (second most common)  any size project wide variety of assays available   good in-depth overview and used for most fecal and soil studies. Good for taxonomy and alpha and beta diversity. Of course, you can increase the depth of coverage e.g. 20K, 40K etc.. the price per sample increases in proportion. A wide variety of assays are currently available for 16s bacteria, archaea, fungi, functional genes etc.

 

5) 1x700bp 454 (batches of at least 40 samples required). good in-depth overview and used for studies requiring longer reads. Good for taxonomy and alpha and beta diversity.   Of course you can increase the depth of coverage e.g. 20K, 40K etc.. the price per sample increases in proportion. A wide variety of assays are currently available for 16s bacteria, archaea, fungi, functional genes etc.

 

6)    15-20,000 avg sequences PGM. Good amount of data and becoming more popular for many types of studies. Of course with this you can option for 20K , 40K etc it just increases price per sample in proportion.   We do have a variety of assays here for 16s, archaea, and functional genes.

 

NOTE:  the prices below are for inhouse assays.. CUSTOM assay prices are generally only an additional $10-$20/barcode (one time setup fee).  If you need a custom assay we are happy to get them ordered and tested.

MRDNA 16s DNA Sequencing Service
MRDNA Bacteria Sequencing Contact Number
MRDNA 16s Sequencing Contact Info
MRDNA 16s DNA Sequencing Service