Can I Submit Raw Sample? How Much Do I Need?
Of course!! MR DNA is glad to offer our customers DNA and RNA Extraction services for almost any sample type e.g. soil, plant matter, feces, etc. Just name it and there is a good chance we have already developed a successful DNA and/or RNA isolation procedure for downstream next-generation sequencing (NGS). This is one of the key advantages of sending MR DNA your raw samples. We know that there are a variety of purification techniques out there, but unfortunately, not all nucleotide purification techniques were made equally. Over the years, MR DNA has had the exciting opportunity to develop extraction methods for a wide range of sample types, and over these years we have made the necessary adjustments to ensure the yield and purity meet the requirements for NGS library prep and sequencing. By allowing MR DNA to take on the task of DNA/RNA isolation, you may also be saving yourself time and money. Aside from the time saved of nucleotide extraction itself, if the DNA or RNA do not meet the necessary quantities or purity requirements for your desired sequencing method then additional services may be required such as linear amplification or additional rounds of column purification to move forward with your project, and thereby increase the time and cost required.
How Much Sample Should I Send?
For DNA sequencing, the amount is dependent upon the type of the sample you have. Soil and tissue samples require at least 0.25g. We recommend sending a few grams extra especially if multiple extraction replicates per sample are requested. As for liquids substrates, 50 milliliters is more than enough to get the job done. Depending on the richness of your substrate, as little as 250ul may be required for the extraction protocol. For RNA sequencing, at least 2 g of raw material is needed. The liquid substrate standards are the same as for DNA extraction.