MRDNA 16s DNA Sequencing Service

Sequencing Services

Sequencing Services

 

MR DNA is a full service next generation sequencing service provider.   If you do not see the application of interest in our sequencing applications (linked to the left) just contact us via phone or email and we will definitely help you with any type of project.

 

examples of our popular sequencing programs

AMPLICON SEQUENCING (bTEFAP® services)

 

examples of amplicon sequencing includes microbiome assays such as 16s ribosomal sequencing,  ITS region for fungi,  18s for general eukaryotes,  functional genes such as nirS, nifH, dsr, rpoB, or ANY other target or custom amplicon.

 

we have several platforms that can be used for amplicons:

 

 

 

 

 

Sequencing Platform Read Length/Assay Length Pricing
Ion Torrent PGM 300bp 515F bacterial + archaeal or ITS1-2 fungal diversity For nominal 15-20,000 reads/assay: $60/assay (any size project)
Ion S5 XL 400-500bp bacterial, archaeal, or ITS fungal diversity assays Same Pricing as Ion Torrent PGM
Ion S5 XL >500bp bacterial, archaeal, or ITS fungal diversity assays Same Pricing as Illumina MiSeq
Illumina MiSeq 2x300 PE 20,000 sequence diversity assays starting at:
$60 /assay (>300 assays)
$65/assay (150-300 assays)
$70/assay (100-150 assays)
$75/assay (50-100 assays)
$80/assay (1-50 assays)
For projects < 10 samples a $100 library fee is added
PacBio Sequel Full Length 16s sequencing Average 5,000 seqeunces per sample: $220/assay (Minimum 20 Samples)

300bp 515F bacterial + archaeal or ITS1-2 fungal diversity PGM (ion torrent) assays with nominal 15-20,000 reads/assay for $60/assay (any size project)

 

Illumina miseq: 2x300bp PE illumina 20,000 sequence diversity assays starting $80/assay 1-50 assays (note: for projects < 10 assays per library, a $100 library fee is added),  $75 for 50-100 assays, 70 for 100-150 assays, $65 for 150-300, $60 for > 300 assays.

 

PAC BIO SEQUEL amplicons.  e.g.  Long read full 16s ~1400bp amplicon diversity average 5k reads per sample (minimum 20 samples) for $220/sample

 

 

Here are examples of Genome and metagenome programs.  Note that we do have bulk discounts for large projects..  these examples are for small projects e.g.  < 5 samples

 

*** Genomes or Metagenome shotgun DNA :

- 2x150bp 10-20 million paired sequences per sample = $500 (data only)

-2x300bp 1-2 million sequences = $600  (data only)

 -2x250bp 10 million sequences = $900(data only)

-2x250bp  4 million sequences = $600 (data only)

 

 

NEW:  gap closure sequencing long read scaffolding = $800/sample

Data assembly with optimization = $200

-----we have all kinds of bioinformatics services for shotgun platforms  just ask ;-)

 

Here are examples for sequencing RNA samples such as RNAseq and metatranscriptomes..  These are small project prices but we do have bulk discounts for large projects.

 

***Transcriptome Metatranscriptome  shotgun RNAseq:

- 2x150bp 10-20 million paired sequences per sample = $600 (data only)

-2x300bp 1-2 million sequences = $700  (data only)

 -2x250bp 10 million sequences = $1000 (data only)

-2x250bp 4 million sequences = $700 (data only)

--- Ribosomal depletion if needed for metatranscrioptomes or bacterial transcriptomes= $400

Communal Genomes or Metagenome:

 

Our new program provides $500 bacterial genomes and metagenomes. This program provides roughly 10 million paired sequences per sample. It is important to note the turnaround for these vary from a few weeks to a few months, therefore this program is only recommended for projects not on a strict deadline.

 

Microbiome Analyses:

 

A free comprehensive taxonomic analysis comes with any 16s, ITS, and 18s data (We are also able to provide analyses for several functional genes as well). Included is a full analysis pipeline comprised of denoising, chimera check, low quality sequence cleaning or removal, clustering, otus generation, taxonomic assignment etc.  Our pipeline generates about 12 different analysis files evaluating the taxonomic data at each taxonomic level:  genus, species, family, class, order, and phylum.  Additionally, OTU files are provided with lineage and identity information as well as other statistical values. To aid in navigating all of the analyses and raw data, a readme help file is provided.

 

For an additional $10/sample (to recover cost only) we perform alpha and beta diversity, rarefaction, unifrac PCoA etc. These analyses are provided raw, but a standard readme help file is provided, making it fairly easy to work through this extensive amount of data.

 

We also offer advanced analysis and report generation. Similar to a research manuscript, the report will contain methods, biostatistics, figures, tables, legends, results section, and findings overview (if relevant).  The cost for advanced analysis and report generation begins at $500 and may increase depending on the size and complexity of the experiment.  Please detail fully your experimental design and the variable information for your samples to give us the ability to accurately quote these costs.  All report generation and other bioinformatics are billed based upon the time required.  We are fast and always make the cost a great value!

 

THE FOLLOWING OPTIONS are also available for targeted amplicon sequencing on each of our platforms:

 

archaea

nifH

HIV

MHC

fungi

protozoa

AND MUCH MORE!

 

 

Our analysis tools are mature for 454, PGM and Illumina.  For functional genes some setup costs may be necessary for analysis to create a taxonomic database.

 

MRDNA 16s DNA Sequencing Service
MRDNA Bacteria Sequencing Contact Number
MRDNA 16s Sequencing Contact Info
Sequencing Platform Read Length/Assay Length Pricing
Ion Torrent PGM 300bp 515F bacterial + archaeal or ITS1-2 fungal diversity For nominal 15-20,000 reads/assay: $60/assay (any size project)
Ion S5 XL 400-500bp bacterial, archaeal, or ITS fungal diversity assays Same Pricing as Ion Torrent PGM
Ion S5 XL >500bp bacterial, archaeal, or ITS fungal diversity assays Same Pricing as Illumina MiSeq
Illumina MiSeq 2x300 PE 20,000 sequence diversity assays starting at:
$60 /assay (>300 assays)
$65/assay (150-300 assays)
$70/assay (100-150 assays)
$75/assay (50-100 assays)
$80/assay (1-50 assays)
For projects < 10 samples a $100 library fee is added
PacBio Sequel Full Length 16s sequencing Average 5,000 seqeunces per sample: $220/assay (Minimum 20 Samples)
MRDNA 16s DNA Sequencing Service