|Sequencing Platform||Read Length/Assay Length||Pricing|
|Ion Torrent PGM||300bp 515F bacterial + archaeal||For nominal 15-20,000 reads/assay:|
|or ITS1-2 fungal diversity||$60/assay (any size project)|
|Ion S5 XL||400-500bp bacterial, archaeal,||Same Pricing as Ion Torrent PGM|
|or ITS fungal diversity assays|
|Ion S5 XL||>500bp bacterial, archaeal,||Same Pricing as Illumina MiSeq|
|or ITS fungal diversity assays|
|Illumina MiSeq||2x300 PE||20,000 sequence diversity assays starting at:|
|$60 /assay (>300 assays)|
|$65/assay (150-300 assays)|
|$70/assay (100-150 assays)|
|$75/assay (50-100 assays)|
|$80/assay (1-50 assays)|
|For projects < 10 samples a $100 library fee is added|
|PacBio Sequel||Low Coverage (< 500 Seqs): $50/assay|
|Medium Coverage (~5,000 Seqs): $150/assay|
|Long Read Amplicon||High Coverage (~10,000 Seqs): $260/assay|
|Sequencing||Custom Coverage available; Pricing based|
|(16s, 18s, ITS, and custom)||on linear increase of 10K seqs pricing|
|For projects < 10 samples a $100 library fee|
|is added & $20/sample additional|
|PCR replication fee|
300bp 515F bacterial + archaeal or ITS1-2 fungal diversity PGM (ion torrent) assays with nominal 15-20,000 reads/assay for $60/assay (any size project)
Illumina miseq: 2x300bp PE illumina 20,000 sequence diversity assays starting $80/assay 1-50 assays (note: for projects < 10 assays per library, a $100 library fee is added), $75 for 50-100 assays, 70 for 100-150 assays, $65 for 150-300, $60 for > 300 assays.
PacBio SEQUEL: Amplicons e.g. Long read full 16s ~1400bp amplicon diversity. Long read amplicon sequencing for 16s, 18s, ITS and custom amplicons. MR DNA now accepts any size project (projects with < 10 samples do have a $100 indexing fee and a $20/sample additional PCR replication fee.)
**Custom assays from 700bp - 3000bp or larger of course have a barcoding fee that is typically $15/barcode
Here are examples of Genome and metagenome programs. Note that we do have bulk discounts for large projects.. these examples are for small projects e.g. < 5 samples
*** Genomes or Metagenome shotgun DNA :
- 2x150bp 10-20 million paired sequences per sample = $500 (data only)
-2x300bp 1-2 million sequences = $600 (data only)
-2x250bp 10 million sequences = $900(data only)
-2x250bp 4 million sequences = $600 (data only)
NEW: gap closure sequencing long read scaffolding = $800/sample
Data assembly with optimization = $200
-----we have all kinds of bioinformatics services for shotgun platforms just ask ;-)
Here are examples for sequencing RNA samples such as RNAseq and metatranscriptomes.. These are small project prices but we do have bulk discounts for large projects.
***Transcriptome Metatranscriptome shotgun RNAseq:
- 2x150bp 10-20 million paired sequences per sample = $600 (data only)
-2x300bp 1-2 million sequences = $700 (data only)
-2x250bp 10 million sequences = $1000 (data only)
-2x250bp 4 million sequences = $700 (data only)
--- Ribosomal depletion if needed for metatranscrioptomes or bacterial transcriptomes= $400
Communal Genomes or Metagenome:
Our new program provides $500 bacterial genomes and metagenomes. This program provides roughly 10 million paired sequences per sample. It is important to note the turnaround for these vary from a few weeks to a few months, therefore this program is only recommended for projects not on a strict deadline.
A free comprehensive taxonomic analysis comes with any 16s, ITS, and 18s data (We are also able to provide analyses for several functional genes as well). Included is a full analysis pipeline comprised of denoising, chimera check, low quality sequence cleaning or removal, clustering, otus generation, taxonomic assignment etc. Our pipeline generates about 12 different analysis files evaluating the taxonomic data at each taxonomic level: genus, species, family, class, order, and phylum. Additionally, OTU files are provided with lineage and identity information as well as other statistical values. To aid in navigating all of the analyses and raw data, a readme help file is provided.
For an additional $10/sample (to recover cost only) we perform alpha and beta diversity, rarefaction, unifrac PCoA etc. These analyses are provided raw, but a standard readme help file is provided, making it fairly easy to work through this extensive amount of data.
We also offer advanced analysis and report generation. Similar to a research manuscript, the report will contain methods, biostatistics, figures, tables, legends, results section, and findings overview (if relevant). The cost for advanced analysis and report generation begins at $500 and may increase depending on the size and complexity of the experiment. Please detail fully your experimental design and the variable information for your samples to give us the ability to accurately quote these costs. All report generation and other bioinformatics are billed based upon the time required. We are fast and always make the cost a great value!
THE FOLLOWING OPTIONS are also available for targeted amplicon sequencing on each of our platforms:
AND MUCH MORE!
Our analysis tools are mature for 454, PGM and Illumina. For functional genes some setup costs may be necessary for analysis to create a taxonomic database.