New Full Length 16s, 18s, and ITS1-4 Sequencing!!!
Now Available 600bp-2500bp Amplicon Sequencing for Improved Diversity Studies
Our Sequencing Platforms
Illumina NovaSeq
The NovaSeq 6000 System is the latest high throughput sequencing instrument released by Illumina. With up to 6Tb of data and 20 Billion paired-end reads made possible by the Illumina NovaSeq...
Illumina MiSeq
Illumina’s MiSeq is quickly becoming the sequencer of choice for researchers, such as yourself, to carryout their genomic and targeted resequencing studies. The highly experienced team at MR DNA is…
Pacbio Sequel
The PacBio Sequel is the newest sequencing platform released by Pacific Biosciences. The PacBio Sequel, aptly named, follows the release of the PacBio RS II System; one of the first to offer read lengths greater than 20Kb, and the PacBio Sequel is no different...
Illumina HiSeq
The HiSeq has been discontinued and all applications moved to the NovaSeq
SeqStudio
Most Sanger methods are now on Pac Bio High Fidelity Long read systems. We feel data is much better and although cost slightly higher it is worth it for most applications.
The 16s rRNA gene for bacteria and archaea, the ITS regions for fungi, the 18s regions for general eukaryote, coi sequencing etc. are the ideal target to complete microbiome studies. MR DNA has extensive arrays of different ribosomal, phylogenetic markers and functional assays in-house
We offer a wide range of NGS platforms, making whole genome sequencing all the more affordable. From small microbial genomes to larger eukaryote genomes, whole genome, RNAseq, transcriptome, isoseq, resequencing, metagenome, metatranscriptome, bisulfite, exome sequencing, target enrichment, reduced representation are just examples of our broad range of services.
MRDNA is passionate about microbiome research. In addition to our 16s sequencing services, ITS sequencing, 18s, COI, rpoB, functional genes or any other type of diversity assay you can imagine or create. we can help you generate the data you need. small project or large project we are your full service end to end solution.
Our Services
Today's Research
Syntroph diversity and abundance in anaerobic digestion revealed through a comparative core microbiome approachAmplicon Sequencing: Uncovering the Depths of Genetic Diversity Precision, Depth, and Insight for Targeted Genomics At MR DNA, our Amplicon Sequencing services are designed to provide detailed insights into specific regions of DNA or RNA, enabling researchers to explore genetic variation, microbial communities, and more with unmatched precision and depth. With our state-of-the-art sequencing technology and experienced team, we empower you to achieve accurate, reproducible results tailored to your specific research needs. What is Amplicon Sequencing? Amplicon sequencing focuses on targeted sequencing of specific genomic regions by amplifying these regions with PCR before sequencing. This approach is highly effective for studying genetic diversity, microbial populations, and variant detection, offering deep insights in a cost-effective and efficient manner. Applications of Amplicon Sequencing Amplicon sequencing is widely used in: Microbiome Studies: Identify and characterize microbial communities by sequencing regions like the 16S rRNA gene (bacteria and archaea), ITS region (fungi), and more. Cancer Research: Detect somatic mutations, gene fusions, and other cancer-related variants by focusing on clinically relevant genomic hotspots. Human Genetics: Study gene panels related to inherited diseases, pharmacogenomics, and other targeted human genome applications. Environmental Genomics: Monitor biodiversity, detect pathogens, and analyze complex ecosystems by sequencing target markers in environmental samples. Agrigenomics: Track genetic traits in plants and animals, enabling breeding programs, pathogen detection, and ecological studies. Our Amplicon Sequencing Services With [Your Company Name], you can expect a complete, streamlined workflow that simplifies your research journey. We offer flexible solutions tailored to your project’s unique goals, from target selection through to data analysis. 1. Customized Amplicon Panels Choose from popular target regions or collaborate with our scientists to design custom amplicon panels that align with your research goals. Our library of primer sets and PCR optimization services ensure precise targeting and high-quality data. 2. High-Resolution Sequencing Using the latest high-throughput sequencing technology, we deliver accurate, high-resolution data, allowing you to identify rare variants, low-abundance microbes, and subtle genetic changes with confidence. 3. Comprehensive Bioinformatics Analysis Our expert bioinformatics team provides comprehensive data analysis, including variant calling, taxonomic classification, diversity analysis, and more. We turn raw sequencing data into actionable insights, enabling you to make discoveries faster. 4. Scalable Solutions for Any Project Size Whether you’re running a small pilot study or a large-scale survey, we offer scalable solutions to fit your project’s size, timeline, and budget. Our fast turnaround times and efficient workflows ensure timely delivery of high-quality data. Why Choose MR DNA for Amplicon Sequencing? High Precision: With optimized protocols and stringent quality controls, we ensure accurate, reproducible results for even the most complex samples. Affordable and Efficient: Amplicon sequencing is a cost-effective approach, providing deep insights without the need for whole-genome sequencing. Expert Guidance: Our scientists and bioinformaticians work with you every step of the way, from panel design to final analysis. Flexible Options: Choose from ready-to-use panels or fully customized solutions to meet your unique research objectives. Secure Data Management: Your data’s security and confidentiality are our top priorities, with robust measures to protect your information at every stage. Start Your Amplicon Sequencing Project Today Whether you’re exploring the human microbiome, monitoring environmental biodiversity, or targeting genetic mutations, [Your Company Name] is here to provide the expertise and tools you need. Discover the power of amplicon sequencing and accelerate your research with confidence. Contact Us Ready to discuss your project? Contact our team today to learn more about our amplicon sequencing services and how we can help you achieve breakthrough results. This content positions your company as a knowledgeable, reliable, and customer-focused provider of amplicon sequencing services, appealing to a broad range of research fields. Let me know if you’d like additional sections or specific details! Unraveling the Blueprint of Life Advanced Technologies for Precise, High-Throughput Sequencing At MR DNA , we specialize in genome sequencing solutions, utilizing the latest in high-throughput sequencing technologies to deliver accurate and detailed insights into the genetic code. Our expertise spans multiple genomic sequencing techniques, allowing researchers and clinicians to explore genetic variations, identify mutations, and unlock new possibilities in fields from healthcare to biotechnology. Our Genome Sequencing Techniques Our genome sequencing services cover a wide range of cutting-edge techniques, ensuring comprehensive data collection and high-resolution analysis. We employ various methods based on project needs, offering precision, efficiency, and scalability: 1. Whole Genome Sequencing (WGS) Whole Genome Sequencing provides a complete map of the genetic material in an organism. This technique covers both coding and non-coding regions of the genome, delivering an in-depth view of genetic variation, structural variants, and mutation profiles. Our WGS services utilize advanced next-generation sequencing (NGS) platforms for fast, accurate, and cost-effective results. Keywords: whole genome sequencing, NGS, genetic variation, structural variants, high-throughput, mutation detection 2. Targeted Sequencing Our targeted sequencing services focus on specific genes or genomic regions, enabling detailed examination of areas associated with diseases, traits, or specific functions. By concentrating on targeted gene panels or customized regions, this method offers efficient, high-depth coverage, ideal for studies in oncology, rare diseases, and pharmacogenomics. Keywords: targeted sequencing, gene panels, custom sequencing, high-depth coverage, oncology, pharmacogenomics, rare diseases 3. Exome Sequencing Whole Exome Sequencing (WES) concentrates on protein-coding regions (exons) to uncover protein-altering mutations linked to diseases. WES is a cost-effective way to focus on the regions that most directly impact phenotypes and disease expression, delivering high-quality data for disease research and clinical applications. Keywords: whole exome sequencing, WES, protein-coding regions, disease expression, cost-effective sequencing, clinical genomics 4. Metagenomic Sequencing Our metagenomic sequencing services identify and classify diverse microorganisms within complex samples, like environmental or human microbiome samples. This powerful technique enables comprehensive analysis of microbial communities without culturing, providing insights into biodiversity, microbial functions, and interactions. Keywords: metagenomic sequencing, microbiome analysis, biodiversity, microbial classification, shotgun sequencing, environmental genomics 5. RNA Sequencing (RNA-Seq) Our RNA sequencing (RNA-Seq) services deliver valuable insights into gene expression and transcriptomics. By examining the transcriptome, researchers can better understand cellular processes, gene regulation, and discover new RNA biomarkers. RNA-Seq is critical for studies in cancer research, developmental biology, and more. Keywords: RNA sequencing, RNA-Seq, transcriptomics, gene expression, transcriptome, biomarker discovery, gene regulation 6. Single-Cell Sequencing Single-cell sequencing allows the analysis of individual cells, revealing cell-specific variations and enabling precision insights into complex tissues and heterogeneous populations. Ideal for studies in oncology, immunology, and neuroscience, single-cell sequencing unveils cellular heterogeneity and rare cell types within a sample. Keywords: single-cell sequencing, cellular heterogeneity, single-cell RNA-Seq, precision genomics, rare cell types, oncology, immunology Applications of Genome Sequencing Genome sequencing has transformative applications across research and clinical fields, empowering advances in: Oncology: Identify cancer mutations, understand tumor heterogeneity, and track clonal evolution with targeted and whole-genome sequencing. Genetic Disease Research: Detect rare genetic mutations and structural variations associated with inherited diseases and syndromes. Microbiome Analysis: Investigate complex microbial ecosystems with metagenomic sequencing for insights into health, agriculture, and environmental science. Pharmacogenomics: Discover genetic variants affecting drug response, enabling personalized medicine and optimized treatment plans. Agrigenomics: Analyze genomes of plants and animals to improve breeding, productivity, and resilience to environmental challenges. Keywords: oncology, cancer genomics, genetic mutations, microbiome analysis, pharmacogenomics, agrigenomics, personalized medicine Why Choose MR DNA ? With extensive experience in genome sequencing technologies, MR DNA provides end-to-end sequencing and bioinformatics support. Our expertise in NGS, WGS, WES, RNA-Seq, and single-cell sequencing allows us to offer tailored solutions to meet your research or clinical objectives. Advanced Platforms: We utilize the latest sequencing technology for high-resolution and high-throughput data. Comprehensive Bioinformatics: Expert analysis, variant calling, pathway analysis, and data visualization ensure meaningful results. Flexible and Scalable Solutions: Customizable workflows for projects of all sizes, from pilot studies to large-scale sequencing efforts. Secure Data Management: Adhering to strict data security protocols to protect your genomic information. Keywords: genome sequencing, next-generation sequencing, bioinformatics, variant calling, data analysis, data security, customized workflows
Anaerobic digestion is an important biotechnology treatment process for conversion of waste to energy. In this study, a comparative core microbiome approach, i.e., determining taxa that are shared in functioning digesters but not shared in non-functioning digesters, was used to determine microbial taxa that could play key roles for effective anaerobic digestion. Anaerobic digester functions were impaired by adding the broad-spectrum antimicrobial triclosan (TCS) or triclocarban (TCC) at different concentrations, and the core microbiomes in both functioning and non-functioning anaerobic digesters were compared. Digesters treated with high (2500 mg/kg) or medium (450 mg/kg) TCS and high (850 mg/kg) TCC concentrations lost their function, i.e., methane production decreased, effluent volatile fatty acid concentrations increased, and pH decreased. Changes in microbial community diversity and compositions were assessed using 16S rRNA gene amplicon sequencing. Microbial richness decreased significantly in non-functioning digesters (p < 0.001). Microbial community compositions in non-functioning digesters significantly differed from those in functioning digesters (p = 0.001, ANOSIM). Microbes identified as potentially key taxa included previously known fatty acid-degrading syntrophs and amino acid-degrading syntrophs. A diverse group of syntrophs detected in this study had low relative abundance in functioning digesters, suggesting the importance of rare microbes in anaerobic digester operation. The comparative microbiome approach used in this study can be applied to other microbial systems where a community-driven biological phenomena can be observed directly.
Fujimoto, M., Carey, D. E., Zitomer, D. H., & McNamara, P. J. (2019). Syntroph diversity and abundance in anaerobic digestion revealed through a comparative core microbiome approach. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-019-09862-4
In The News
Agrigenomics
Microbial Genomics
Clinical Health
Microbial genome sequencing is helping to improve our understanding of human health, disease, and microbial evolution. The human body contains trillions of cells with a variety of microbes that play a critical role in human health and disease, but the area of mechanism remains a mystery. Microbes are not only present in the human body; they are everywhere e.g. human or animal guts, homes, plants, oceans, and soil. Microbial research has gone under-appreciated for a long time, but with the help of next-generation sequencing (NGS), scientists are now investigating this vast microbial world. Multiple studies have been published in the last 5-10 years examining the microbial communities that exist inside our bodies and how these microbiomes
The one bacteria most everyone is familiar with, and maybe without even knowing they are...Clostridium difficile. This diarrhea causing bacteria, C. difficile, may be splitting into two. Researchers from the Wellcome Sanger Institute have just completed a large scale research project evaluating this pesky little bug with specific concern to its presence within hospital environments. This study involved sequencing the DNA of 906 strains of C. difficile collected across 33 countries, and as a result of this collective effort, scientists suggest that a new bacterial species is emerging, which is currently known as C. difficile clade A.
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In The News
16s Sequencing
16s sequencing is one of the go to sequencing methods to complete microbiome studies. 16s sequencing involves sequencing of the 16s rRNA gene found in all bacteria and archaea. The fact that the 16s rRNA gene can be found in all prokaryotes make the 16s gene the ideal candidate to characterize the microbiome of numerous environments. Another key factor that makes the 16s gene an ideal candidate for microbiome characterization is the fact that there are both highly conserved regions and highly variable regions within the gene. The highly conserved regions within the 16s rRNA gene make great targets for PCR primers bind to and replicate thereby producing millions of copies of the targeted hypervariable region that is flanked by the highly conserved regions. The 16s rRNA gene contains 9 hypervariable regions that can be targeted in order to gain insight into the diversity amongst prokaryotes.
16s rRNA sequencing has grown in popularity over the last decade due in large part to next-generation sequencing. NGS, also known as high-throughput sequencing, has drastically reduced the cost of DNA sequencing due to its capability to sequence hundreds of samples at a time. In the past, capillary electrophoresis was able to produce ~1Mb per run, and today, with instruments such as the Illumina HiSeq, we are able to produce ~500 Gb per run. Until recently, one metric that Sanger sequencing had as an advantage over NGS instruments such as the Illumina MiSeq and Ion Torrent was read length. On average, capillary electrophoresis was able to achieve read lengths up to 1,000bp in comparison to the 400-600bp read lengths provided by Illumina and Ion Torrent. However, the PacBio Sequel now allows researchers the ability to achieve read lengths ranging from 10-30Kb. What does this mean for 16s rRNA sequencing? We can now sequence the entire 16s rRNA gene, which is approximately 1.5Kb in length. Whether your goal is to target one specific hypervarible region or the entire 16s rRNA gene, there is a NGS platform for you.
Metagenome Sequencing
Metagenome sequencing is a term that can cause a lot of confusion, as well as bring so much joy to microbiologists around the world. Metagenome sequencing is a sequencing method that investigates the DNA extracted from an environmental sample as a whole. Whereas certain DNA sequencing techniques will target a specific organism from a specific environment, metagenome sequencing targets all microbial organisms found in a certain environment. There are predominantly two methods used to completely metagenomics studies, 16s rRNA sequencing and Shotgun metagenome sequencing. The difference between the two sequencing methods can be found in their names. 16s rRNA sequencing, as you would expect, targets only the 16s rRNA gene, while Shotgun metagenome sequencing targets all genes present in your sample. How is this accomplished?
Shotgun metagenome sequencing involves the random shearing of all DNA present in a particular sample. These smaller fragments are sequenced on NGS platforms such the Illumina MiSeq and then reassembled. Because shotgun metagenomics is non-discriminatory, not only are you able to gain taxonomic information, similar to 16s sequencing, but you are also able to gain insight to the presence of functional genes as well. Overall, shotgun metagenome sequencing provides a more complete picture of your environmental sample. NGS platforms such as the Illumina HiSeq allow researchers to ability to sequence their metagenome samples to a much greater depth. Why is sequencing depth important? Naturally, the most abundant organisms in your sample will receive the most amounts of data, and because you are not specifically targeting certain organisms, without sufficient sequencing coverage, you run the risk of not identifying those organisms that may be underrepresented. From the human microbiome, to pond water, and to the rumen of cattle, shotgun metagenome sequencing paired with NGS platforms has the ability to take your ecological study to the next level.
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