De novo sequencing

Overview

Shotgun sequencing and de novo assembly of genomes or transcriptomes at MR DNA.

Reference assembly and long read genomes

MR DNA has many programs for sequencing DNA and RNA (RNAseq, metagenomes, metatranscriptomes, eukaryote genomes, prokaryote transcriptomes, bacterial genomes, virus, phage, plasmid, fosmid etc.

THIS LIST of programs is for small projects and minimum scale sequencing, with only a few samples, but definitely with larger numbers of samples or samples requiring much more data we definitely can help and provide volume and bulk discounting for some of the lowest price fastest turnaround commercial, academic and government rates available anywhere!

here are just a few examples of shotgun sequencing programs but do contact us for customized projects especially if you have more than a few samples or need larger amounts of data .. we are here to work hard and help!

NOTE:*** Pricing below is for small projects 1-2 samples discounts start at 3-5 samples and of course large and bulk discounts available for larger projects ..please email us for accurate pricing

Example small‑project programs (legacy)

• - 2x150bp 10-20 million paired sequences per sample = $300 (data only) Illumina NovaSeq
• - 2x300bp 1-2 million sequences = $500 (data only) Illumina NovaSeq
• - 2x250bp 10 million sequences = $500 (data only) Illumina NovaSeq
• - 2x250bp 4 million sequences = $350 (data only) Illumina NovaSeq
• - 2x150bp 10-20 million paired sequences per sample = $300 (data only)

RNA / transcriptome examples

• MR DNA has many programs for sequencing DNA and RNA (RNAseq, metagenomes, metatranscriptomes, eukaryote genomes, prokaryote transcriptomes, bacterial genomes, virus, phage, plasmid, fosmid etc.
• ***Transcriptome, Metatranscriptome, RNAseq, polyA and shotgun total RNA :
• --- Ribosomal depletion (new lower pricing) if needed for metatranscriptomes or bacterial transcriptomes= $100
• BIOINFORMATICS FOR transcriptome or other RNA based projects
• usual costs for comparing two groups with RNAseq is $100
• DNA Extraction / RNA Extraction Price:
• -- RNA Extraction starting between $50 - $80 / sample (depending on service requested and sample type submitted)

Bioinformatics options

• Shotgun sequencing and de novo assembly of genomes or transcriptomes at MR DNA.
• NEW: gap closure sequencing long read scaffolding for bacteria = $700/sample.. this adds long read data to Illumina sequencing data and may result in much fewer contigs during hybrid assembly.
• BIOINFORMATICS FOR GENOMES
• Data assembly with optimization annotation = $100 (for bacterial genomes we also provide free genome annotation giving you genbank files, proteome files, transcriptome files and genome spreadsheets)
• BIOINFORMATICS FOR transcriptome or other RNA based projects
• usual costs for annotation of a transcriptome with assembly is $100
• It is well known that next generation sequencing applications have led to numerous genomic discoveries that would otherwise have been improbable if not impossible. However, the number of genomes that have yet to be discovered is countless. The identity of these novel genomes is elucidated through De Novo Sequencing. Sequencing and assembly of novel genomes is only made possible with sufficient coverage of the genome of interest along with extended read lengths. While NGS has always offered researches deep sequencing capabilities, as the read length capabilities of next generation sequencers steadily increase, not only does De Novo Sequencing become more reliable, but it is also becomes more affordable. Integrating a seasoned laboratory staff with a bioinformatics team that has 20 years of continuous applied bioinformatics experience allows MR DNA to offer its customers a low cost sequencing solution, not only with regards to reference genomes, but novel genomes as well.
• Our bioinformatics pipeline is designed to streamline genome assembly, annotation, and analysis. For de novo genomes, we provide tools for scaffolding, gap closure, and repeat resolution. Reference genome projects benefit from precise read alignment, variant calling, and structural annotation. Whether your focus is on agricultural improvements, biomedical research, or environmental applications, Molecular Research MR DNA is committed to delivering high-quality genome sequencing solutions. With state-of-the-art technology and expert support, we help you unlock the potential of genomic data to drive innovation and discovery.

Extraction pricing (starting ranges)

• DNA Extraction / RNA Extraction Price:
• -- DNA Extraction starting at $40 / sample (depending on service requested and sample type submitted)
• -- RNA Extraction starting between $50 - $80 / sample (depending on service requested and sample type submitted)

Custom projects

Pricing above reflects legacy small-project examples. For accurate totals (and discounts for 3+ samples or larger datasets), request a quote and we’ll recommend the most efficient platform and deliverables.