Skip to content
Call806-789-7984 Emailinfo@mrdnalab.com Request a Quote

Sequencing Services

Bisulfite sequencing for DNA methylation analysis

Measure cytosine methylation at base resolution using bisulfite conversion and high-throughput sequencing. MR DNA supports projects from small studies to large programs, including WGBS and RRBS.

Request a Quote Bioinformatics Options
Methods
WGBS • RRBS
Outputs
FASTQ • QC • Reports
Scope
Single studies to large cohorts

Service options

Choose the approach that fits your biological question, genome size, and budget.

Dont forget the benefits of Pac Bio Sequel  PacBio Sequel IIe directly detects multiple DNA modifications on native long reads—no bisulfite conversion required—linking epigenetics, structure, and haplotype in a single assay.

Whole-genome bisulfite sequencing (WGBS)

Genome-wide methylation profiling for comprehensive methylome analysis.

  • Base-resolution methylation calls across the genome
  • Best for discovery and broad regulatory analyses
  • Supports differential methylation workflows
  • Bisulfite sequencing is the gold-standard method for genome-wide DNA methylation analysis, providing single-base resolution of cytosine methylation across regulatory and coding regions. By chemically converting unmethylated cytosines while preserving methylated sites, bisulfite sequencing enables precise quantification of methylation patterns that drive gene regulation, development, and disease. MR DNA offers scalable bisulfite sequencing workflows—including WGBS and RRBS—with optional bioinformatics for methylation calling and differential methylation analysis. 

Reduced representation bisulfite sequencing (RRBS)

Enriched coverage of CpG-rich regions for efficient methylation profiling.

  • Targets CpG islands and promoter-rich regions
  • Cost-effective for cohorts and comparative studies
  • Project-dependent coverage and design
  • PacBio Sequel IIe enables direct detection of DNA base modifications without bisulfite conversion, preserving native DNA and avoiding chemical degradation or GC bias. Using single-molecule real-time (SMRT) sequencing, the platform detects multiple epigenetic marks—including 5-methylcytosine (5mC), 6-methyladenine (6mA), and 4-methylcytosine (4mC)—by measuring polymerase kinetics across long reads. This allows simultaneous analysis of sequence, structure, haplotype, and epigenetic modification on the same DNA molecule, providing long-range, phase-resolved methylation insights that short-read bisulfite methods cannot capture. 

What you receive

  • Demultiplexed FASTQ files
  • Run-level and sample-level QC summaries
  • Optional methylation calling and summary tables
  • Optional differential methylation analysis (study-design dependent)
  • Methods documentation and reporting (optional)

What to include in a quote request

  • Organism / genome size
  • Sample count and grouping (controls vs treatment, timepoints)
  • Preferred method: WGBS or RRBS (or ask us to recommend)
  • Desired deliverables (raw data only vs analysis/report)
  • Timeline requirements
Request a Quote Email a scientist

Ready to start a methylation project?

Send your sample counts and study goals. We’ll recommend an efficient bisulfite sequencing plan and provide a quote.

Request a Quote Bioinformatics