De novo genome assembly
Long reads improve contiguity and resolve repeats, yielding higher-quality assemblies.
De novo sequencing →PacBio Sequel
Long-read sequencing for complex genomes and transcripts—ideal for de novo assembly, structural variants, full-length amplicons, and Iso-Seq transcript discovery.
Best for
Complex regions & assemblies
Strength
Long reads / high contiguity
Common outputs
Assemblies • SVs • Isoforms
Structural variant detection
Resolve large insertions, deletions, inversions, and complex rearrangements.
Genome sequencing →Iso-Seq transcriptomics
Full-length isoform sequencing for alternative splicing and transcript discovery.
Transcriptome sequencing →Full-length amplicons
High-accuracy long amplicons (e.g., full-length 16S/18S/ITS and custom targets).
Amplicon sequencing →Complex regions
Resolve repetitive or GC/AT-biased regions where short reads struggle.
Bioinformatics →Hybrid strategies
Combine short-read and long-read data to optimize accuracy and contiguity.
NovaSeq 6000 →When to choose long reads
- Your assembly has too many contigs or unresolved repeats
- You need structural variants or haplotype resolution
- You need full-length transcripts or isoform-level precision
- You are validating complex edits or structural changes
What to include in a request
- Organism / genome size (if known)
- Sample count and sample type
- Goal (assembly / SVs / Iso-Seq / long amplicon)
- Desired deliverables (FASTQ, assembly, variants, report)
- Timeline
Additional platform notes
Sequencing Platforms
Single-molecule real-time sequencing delivering ultra-long reads for complex genomes and transcriptomes.
PacBio Sequel excels where short-read technologies struggle, including repetitive regions and complex genomic architecture.
Use long-read sequencing for your project
Tell us your target, sample count, and goals—we’ll recommend the best long-read or hybrid plan.