Illumina AmpliSeq Targeted Sequencing
Highly multiplexed targeted DNA and RNA sequencing for efficient detection of variants, biomarkers, and gene panels. Illumina AmpliSeq workflows support focused studies where depth, speed, and cost control matter.
For fastest scoping, send: gene list/panel name, organism, sample type, number of samples, and whether you need sequencing only or bioinformatics/variant outputs.
When to Use Illumina AmpliSeq
AmpliSeq is ideal when you want deep coverage of a defined target set—genes, hotspots, or transcripts—without the cost and complexity of whole genome or whole transcriptome sequencing.
Common Panel Types
- Cancer panels for hotspot variants, pathway genes, and focused biomarker studies
- Inherited disease panels targeting clinically relevant gene sets for research use
- Microbial targets for strain markers, resistance genes, or defined loci
- Custom gene panels designed to match your project’s hypotheses
Advantages for Focused Studies
- High multiplexing enables many targets in a single workflow
- Deep coverage improves sensitivity for low-frequency variants (study-dependent)
- Cost control by restricting sequencing to relevant targets
- Flexible outputs from data-only to analyzed variant deliverables
Deliverables & Optional Bioinformatics
Choose sequencing-only or a complete analysis package tailored to your study goals.
Data Deliverables
- FASTQ files and QC summaries
- Coverage metrics across targets (when applicable)
- Optional alignment files (BAM/CRAM)
Analysis Options
- Variant calling for SNPs/indels (scope-dependent)
- Target metrics: on-target rate, depth distribution, uniformity
- VCF outputs and annotated variant tables (when requested)
Project Scoping Support
- Panel selection or design input (custom targets)
- Depth planning based on targets and sample count
- Deliverables aligned to your downstream pipeline
Illumina AmpliSeq FAQ
Quick answers to help plan a targeted sequencing project.
Can you run custom panels or only standard designs?
We can support both off-the-shelf panels and custom target sets. Share your gene list or target coordinates and organism, and we’ll recommend a workflow and deliverables.
Do you support both DNA and RNA targeted panels?
Yes—projects can be scoped for targeted DNA sequencing and targeted RNA panels depending on goals and sample type.
What do you need to quote a project?
Panel name or gene list, organism, sample type, number of samples, target depth goals, and whether you need sequencing only or bioinformatics outputs.
Start an Illumina AmpliSeq project
Share your panel name or gene list and sample details. We’ll recommend a workflow and deliverables.