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Platforms

Illumina HiSeq 2500

High-throughput short-read sequencing platform. Note: replaced by NovaSeq 6000 at MR DNA for most applications.

Platform replaced For most projects, MR DNA now runs short-read work on Illumina NovaSeq 6000 for higher throughput and better efficiency.

Historical HiSeq use cases

  • Whole genome sequencing (short-read)
  • RNA-seq and transcriptomics
  • Large multiplexed studies
  • Legacy workflow compatibility

Why NovaSeq 6000 now

  • Higher output per run
  • Better cost per base at scale
  • Modern chemistry and run modes

What to include in a request

  • Sample type and count
  • Target data volume (reads per sample / coverage)
  • Read length preference (e.g., 2×150)
  • Deliverables (FASTQ only vs analysis/report)

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